DOT Drug Testing

DOT Drug Testing in Chicago

Walk-ins welcome! We handle everything from pre-employment to return-to-duty, all in one place.

From Individual Drivers To Full Fleets, We Provide DOT Drug Testing That Fits Your Needs. Contact Us To Get Started

What Is A DOT Drug Test?

Types of DOT Drug Tests We Offer

Pre-Employment

Required before a driver begins safety-sensitive duties to ensure they are drug-free and eligible to operate.

Post-Accident

Conducted after qualifying incidents to determine if drugs or alcohol may have contributed to the event.

Random

Unannounced testing throughout the year as part of a compliant random program for drivers.

Reasonable Suspicion

Performed when a trained supervisor observes signs of possible drug or alcohol use.

Return-to-Duty

Required after a failed test, once a Substance Abuse Professional (SAP) has cleared the driver to resume work.

Follow-Up

Ongoing testing after return-to-duty to ensure continued compliance.

Who We Work With

We work with drivers and employers at every stage of the DOT process—from getting started to getting back on the road.

What To Expect When You Go For Your DOT Drug Test

Step 1 - Check In With I.D

Bring a valid photo ID and complete a brief check-in.

Step 2 - Review The Process

You’ll be guided through the DOT testing procedure and what to expect.

Step 3 - Prepare For Collection

Personal items (bags, jackets, etc.) are set aside before providing your sample.

Step 4 - Provide Your Sample

You’ll provide a urine sample in a private setting (unless observation is required).

Step 5 - Sample Handling & Sealing

The sample is checked, split into two bottles, and sealed—while remaining in your sight at all times.

Step 6 - Paperwork & Completion

You’ll review and sign the required forms and receive a copy for your records.

Walk In Or Schedule Your Appointment—We’ll Make The Process Fast, Simple, And Compliant

Frequently Asked Questions

Why Does A DOT 5-Panel Drug Test Have More Than 5 Substances Tested?

Please note that once a DOT collection is started, it needs to be completed. There is no “coming back later”; not complying with testing requirements may be considered a refusal to test.

This occurs when an employee subject to DOT regulations refuses to undergo a required drug test. This is treated as a violation under DOT regulations.

Refusal to test can include:

  1. Explicit Refusal: When an employee outright refuses to submit to a drug test when directed to do so.

  2. Failure to Provide Adequate Sample: If an employee fails to provide an adequate urine sample for drug testing without a valid medical explanation.

  3. Failure to Cooperate with the Testing Process: This can include actions such as not following instructions, leaving the testing site without permission, or behaving in a way that obstructs the testing process.

Failure to Remain at the Testing Site: Leaving the testing site before the testing process is complete without a valid reason.

  • Negative lab-based results usually take 1-2 business days from the time the lab receives the sample. 
  • Positive results can take as long as 5-7 days to be reported.

During this collection, the trained observer (who is of the same gender as the donor) will accompany the individual to the restroom and visually monitor the urination process to ensure that the sample is not tampered with or substituted. This is most commonly done during the return-to duty process.

Testing positive triggers a series of consequences and procedures that includes:

  • Employer notification and immediate removal from safety-sensitive duties.

  • Evaluation by a substance abuse professional (SAP) and recommendation of education/treatment program. 

  • Successful completion of SAP treatment program.

  • Providing a negative return-to-duty drug test.

  • Submitting to unannounced follow-up drug testing for a specified period as determined by the SAP.

Additional Services

DOT Physicals

CDL and some non-CDL drivers must successfully undergo a DOT physical to ensure they meet the necessary health standards for operating commercial vehicles.

Step 5 / Return-To-Duty Testing

RTD testing support, including scheduling, lab coordination, and Clearinghouse updates to help drivers complete the SAP process smoothly.

Consortium

We provide consortium services for DOT regulated companies and independent drivers, ensuring you maintain compliance with DOT drug and alcohol random testing requirements.

Book Your DOT Drug Test

Find a Testing Location Near You

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